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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | NADH:ubiquinone oxidoreductase subunit A7 |
| Gene Symbol: | NDUFA7 |
| Synonyms: | B14.5a; CI-B14.5a; A7 |
| Gene ID: | 4701 |
| UniProt ID: | O95182 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.64 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NDUFA7 polyclonal antibody |
Background
This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain.
Images
 | Western blot analysis of various lysates using [KO Validated] NDUFA7 Rabbit pAb (A8441) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type (WT) and NDUFA7 knockout (KO) 293T cells, using [KO Validated] NDUFA7 Rabbit pAb (A8441) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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