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KO-Validated NDUFC2 Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | NADH:ubiquinone oxidoreductase subunit C2 |
| Gene Symbol: | NDUFC2 |
| Synonyms: | HLC-1; B14.5b; MC1DN36; NADHDH2; CI-B14.5b; C2 |
| Gene ID: | 4718 |
| UniProt ID: | O95298 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NDUFC2 (NP_004540.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.84 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NDUFC2 polyclonal antibody |
Background
Involved in mitochondrial respiratory chain complex I assembly. Located in mitochondrion. Part of mitochondrial respiratory chain complex I. Implicated in nuclear type mitochondrial complex I deficiency.
Images
 | Western blot analysis of various lysates using [KO Validated] NDUFC2 Rabbit pAb (A15073) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Western blot analysis of lysates from wild type (WT) and NDUFC2 knockout (KO) HeLa cells, using [KO Validated] NDUFC2 Rabbit pAb (A15073) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
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