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| Reactivity: | Human |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | sirtuin 1 |
| Gene Symbol: | SIRT1 |
| Synonyms: | SIR2; SIR2L1; SIR2alpha; T1 |
| Gene ID: | 23411 |
| UniProt ID: | Q96EB6 |
| Clone ID: | 8E2I5 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 648-747 of human SIRT1 (Q96EB6). |
| Dilution: | WB 1:1000-1:4000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SIRT1 monoclonal antibody |
Background
The gene SIRT1 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from wild type (WT) and SIRT1 knockout (KO) HeLa cells using [KO Validated] SIRT1 Rabbit mAb (A19667) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of HeLa cells using [KO Validated] SIRT1 Rabbit mAb (A19667). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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