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| Reactivity: | Human |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | staphylococcal nuclease and tudor domain containing 1 |
| Gene Symbol: | SND1 |
| Synonyms: | p100; TDRD11; Tudor-SN |
| Gene ID: | 27044 |
| UniProt ID: | Q7KZF4 |
| Clone ID: | 4C8Y3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-285 of human SND1 (NP_055205.2). |
| Dilution: | WB 1:3000-1:18000; IHC 1:500-1:5000; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1.86 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SND1 monoclonal antibody |
Background
The gene SND1 encodes a transcriptional co-activator that interacts with the acidic domain of Epstein-Barr virus nuclear antigen 2 (EBNA 2), a transcriptional activator that is required for B-lymphocyte transformation. Other transcription factors that interact with this protein are signal transducers and activators of transcription, STATs. This protein is also thought to be essential for normal cell growth. A similar protein in mammals and other organisms is a component of the RNA-induced silencing complex (RISC).
Images
 | Western blot analysis of lysates from wild type (WT) and SND1 knockout (KO) HeLa cells using [KO Validated] SND1 Rabbit mAb (A25813) at 1:3700 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from Jurkat cells using [KO Validated] SND1 Rabbit mAb (A25813) at 1:3700 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using [KO Validated] SND1 Rabbit mAb (A25813) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Confocal imaging of HeLa cells using [KO Validated] SND1 Rabbit mAb (A25813, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of NIH/3T3 cells using [KO Validated] SND1 Rabbit mAb (A25813, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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