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Reactivity: | Human |
Applications: | WB, IHC-P, IF/ICC, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | annexin A1 |
Gene Symbol: | ANXA1 |
Synonyms: | ANX1; LPC1; A1 |
Gene ID: | 301 |
UniProt ID: | P04083 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1). |
Dilution: | WB 1:2000-1:10000; IF/IC 1:100-1:2000 |
Purification Method: | Affinity purification |
Concentration: | 0.92 mg/ml |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-ANXA1 polyclonal antibody |
Background
The gene ANXA1 encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.
Images
![]() | Western blot analysis of lysates from wild type (WT) and ANXA1 knockout (KO) HeLa cells, using [KO Validated] ANXA1 Rabbit pAb (A1118) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![]() | Western blot analysis of various lysates, using [KO Validated] ANXA1 Rabbit pAb (A1118) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human spleen using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunofluorescence analysis of A-431 cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunofluorescence analysis of A-549 cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunofluorescence analysis of HeLa cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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