KO-Validated ANXA1 Rabbit pAb (20 μl)

KO-Validated ANXA1 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A1118
Availability: In Stock
$128.00
-+
Reactivity: Human    
Applications: WB, IHC-P, IF/ICC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: annexin A1
Gene Symbol: ANXA1
Synonyms: ANX1; LPC1; A1
Gene ID: 301
UniProt ID: P04083
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1).
Dilution: WB 1:2000-1:10000; IF/IC 1:100-1:2000
Purification Method: Affinity purification
Concentration: 0.92 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol ,pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-ANXA1 polyclonal antibody




Background

The gene ANXA1 encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.




Images

ANXA1 rabbit pAbWestern blot analysis of lysates from wild type (WT) and ANXA1 knockout (KO) HeLa cells, using [KO Validated] ANXA1 Rabbit pAb (A1118) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ANXA1 polyclonal antibodyWestern blot analysis of various lysates, using [KO Validated] ANXA1 Rabbit pAb (A1118) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human spleen using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of A-431 cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A-549 cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using [KO Validated] ANXA1 Rabbit pAb (A1118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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