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KO-Validated Annexin A5/Annexin V Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | annexin A5 |
| Gene Symbol: | ANXA5 |
| Synonyms: | PP4; ANX5; ENX2; CPB-I; RPRGL3; HEL-S-7; VAC-alph; V |
| Gene ID: | 308 |
| UniProt ID: | P08758 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human Annexin A5/Annexin V (NP_001145.1). |
| Dilution: | WB 1:1000-1:4000; IHC 1:1000-1:4000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.78 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ANXA5 polyclonal antibody |
Background
The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications.
Images
 | Western blot analysis of various lysates using [KO Validated] Annexin A5/Annexin V Rabbit pAb (A1728) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Western blot analysis of lysates from wild type (WT) and Annexin A5/Annexin V knockout (KO) 293T cells, using [KO Validated] Annexin A5/Annexin V Rabbit pAb (A1728) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HeLa cells using [KO Validated] Annexin A5/Annexin V Rabbit pAb (A1728) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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