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KO-Validated RhoGDI Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Rho GDP dissociation inhibitor alpha |
| Gene Symbol: | ARHGDIA |
| Synonyms: | GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e; DI |
| Gene ID: | 396 |
| UniProt ID: | P52565 |
| Clone ID: | 0D6X5 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 70-150 of human RhoGDI (P52565). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.10 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ARHGDIA monoclonal antibody |
Background
The gene ARHGDIA encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using RhoGDI Rabbit mAb (A11556) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type (WT) and RhoGDI knockout (KO) 293T cells, using [KO Validated] RhoGDI Rabbit mAb (A11556) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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