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KO-Validated ARHGEF1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Rho guanine nucleotide exchange factor 1 |
| Gene Symbol: | ARHGEF1 |
| Synonyms: | LSC; GEF1; IMD62; LBCL2; SUB1.5; P115-RHOGEF; F1 |
| Gene ID: | 9138 |
| UniProt ID: | Q92888 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.90 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ARHGEF1 polyclonal antibody |
Background
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Images
 | Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit pAb (A4274) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and ARHGEF1 knockout (KO) 293T cells, using [KO Validated] ARHGEF1 Rabbit pAb (A4274) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] ARHGEF1 Rabbit pAb (A4274) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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