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KO-Validated Carbonic Anhydrase 2 (CA2) Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | carbonic anhydrase 2 |
| Gene Symbol: | CA2 |
| Synonyms: | CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282; 2) |
| Gene ID: | 760 |
| UniProt ID: | P00918 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Carbonic Anhydrase 2 (CA2) (NP_000058.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.39 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CA2 polyclonal antibody |
Background
The protein encoded by the gene CA2 is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and Carbonic Anhydrase 2 (CA2) knockout (KO) 293T cells, using [KO Validated] Carbonic Anhydrase 2 (CA2) Rabbit pAb (A18034) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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