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KO-Validated Calpain 2 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | calpain 2 |
| Gene Symbol: | CAPN2 |
| Synonyms: | CANP2; mCANP; CANPL2; CANPml; 2 |
| Gene ID: | 824 |
| UniProt ID: | P17655 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human Calpain 2 (NP_001739.2). |
| Dilution: | WB 1:500-1:3000; IHC 1:200-1:2000; IF/IC 1:50-1:400 |
| Purification Method: | Affinity purification |
| Concentration: | 2.02 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CAPN2 polyclonal antibody |
Background
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and Calpain 2 knockout (KO) 293T cells, using [KO Validated] Calpain 2 Rabbit pAb (A1861) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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