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KO-Validated PRMT4/CARM1 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | coactivator associated arginine methyltransferase 1 |
| Gene Symbol: | CARM1 |
| Synonyms: | PRMT4; M1 |
| Gene ID: | 10498 |
| UniProt ID: | Q86X55 |
| Clone ID: | 4Y3I1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 486-603 of human PRMT4/CARM1 (NP_954592.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.9 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CARM1 monoclonal antibody |
Background
The gene CARM1 belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.
Images
 | Western blot analysis of lysates from MCF7 cells, using [KO Validated] PRMT4/CARM1 Rabbit mAb (A21106) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates using [KO Validated] PRMT4/CARM1 Rabbit mAb (A21106) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type (WT) and PRMT4/CARM1 knockout (KO) 293T cells, using [KO Validated] PRMT4/CARM1 Rabbit mAb (A21106) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg PRMT4/CARM1 antibody (A21106). Western blot was performed from the immunoprecipitate using PRMT4/CARM1 antibody (A21106) at a dilution of1:1000. |
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