KO-Validated HP1 gamma/CBX3 Rabbit pAb (20 μl)

KO-Validated HP1 gamma/CBX3 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A2248
Availability: In Stock
$128.00
-+
Reactivity: Human    
Applications: WB, IHC, IF/IC, IP, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: chromobox 3
Gene Symbol: CBX3
Synonyms: HECH; HP1-GAMMA; HP1Hs-gamma; X3
Gene ID: 11335
UniProt ID: Q13185
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human HP1 gamma/HP1 gamma/CBX3 (NP_009207.2).
Dilution: WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 0.42 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol ,pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-CBX3 polyclonal antibody




Background

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by the gene CBX3 binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.




Images

CBX3 rabbit pAbWestern blot analysis of various lysates using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
HP1 gamma rabbit pAbWestern blot analysis of lysates from wild type (WT) and HP1 gamma/HP1 gamma/CBX3 knockout (KO) 293T cells, using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
CBX3 polyclonal antibodyImmunofluorescence analysis of C6 cells using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Anti-CBX3 antibodyImmunofluorescence analysis of U-2 OS cells using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg HP1 gamma/CBX3 antibody (A2248). Western blot was performed from the immunoprecipitate using HP1 gamma/CBX3 antibody (A2248) at a dilution of 1:1000.



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