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| Reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | cyclin dependent kinase 2 |
| Gene Symbol: | CDK2 |
| Synonyms: | CDKN2; p33(CDK2); K2 |
| Gene ID: | 1017 |
| UniProt ID: | P24941 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-298 of human CDK2 (NP_001789.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:100; IF/IC 1:10-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 3.05 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CDK2 polyclonal antibody |
Background
This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from wild type (WT) and CDK2 knockout (KO) 293T cells, using [KO Validated] CDK2 Rabbit pAb (A18000) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] CDK2 Rabbit pAb (A18000) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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