KO-Validated NG2/CSPG4 Rabbit pAb (20 μl)

KO-Validated NG2/CSPG4 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A3592
Availability: In Stock
$128.00
-+
Reactivity: Human    
Applications: WB, IHC, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: chondroitin sulfate proteoglycan 4
Gene Symbol: CSPG4
Synonyms: NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG; G4
Gene ID: 1464
UniProt ID: Q6UVK1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2).
Dilution: WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 0.69 mg/mL
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-CSPG4 polyclonal antibody




Background

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.




Images

CSPG4 rabbit pAbWestern blot analysis of lysates from A375 cells, using [KO Validated] NG2/CSPG4 Rabbit pAb (A3592) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
CSPG4 polyclonal antibodyWestern blot analysis of various lysates using [KO Validated] NG2/CSPG4 Rabbit pAb (A3592) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
NG2 polyclonal antibodyWestern blot analysis of various lysates, using NG2/CSPG4 Rabbit pAb (A3592) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): MCF7
Exposure time: 30s.



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