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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | DNA damage inducible transcript 4 |
| Gene Symbol: | DDIT4 |
| Synonyms: | Dig2; REDD1; REDD-1; T4 |
| Gene ID: | 54541 |
| UniProt ID: | Q9NX09 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.72 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-DDIT4 polyclonal antibody |
Background
Predicted to enable 14-3-3 protein binding activity. Involved in defense response to virus; negative regulation of TOR signaling; and response to hypoxia. Located in cytosol.
Images
 | Western blot analysis of lysates from wild type (WT) and DDIT4 knockout (KO) HeLa cells, using [KO Validated] DDIT4 Rabbit pAb (A8086) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Western blot analysis of various lysates using [KO Validated] DDIT4 Rabbit pAb (A8086) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
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