Your shopping cart is empty!
KO-Validated DNAJA1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | DnaJ heat shock protein family (Hsp40) member A1 |
| Gene Symbol: | DNAJA1 |
| Synonyms: | DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSJ-2; HSPF4; NEDD7; hDJ-2; A1 |
| Gene ID: | 3301 |
| UniProt ID: | P31689 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 178-397 of human DNAJA1 (NP_001530.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.48 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-DNAJA1 polyclonal antibody |
Background
The gene DNAJA1 encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes.
Images
_KO-WB_01.jpg?t=1709190856) | Western blot analysis of lysates from wild type (WT) and DNAJA1 knockout (KO) 293T cells, using [KO Validated] DNAJA1 Rabbit pAb (A19865) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
You may also be interested in:
