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KO-Validated HuR/ELAVL1 Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ELAV like RNA binding protein 1 |
| Gene Symbol: | ELAVL1 |
| Synonyms: | HUR; Hua; MelG; ELAV1; L1 |
| Gene ID: | 1994 |
| UniProt ID: | Q15717 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR/ELAVL1 (NP_001410.2). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.31 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ELAVL1 polyclonal antibody |
Background
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.
Images
 | Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of MCF-7 cells using [KO Validated] HuR/ELAVL1 Rabbit pAb (A1608). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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