KO-Validated HER2/ErbB2 Rabbit pAb (20 μl)

KO-Validated HER2/ErbB2 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A21768
Availability: In Stock
$128.00
-+
Reactivity: Human    
Applications: WB, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: erb-b2 receptor tyrosine kinase 2
Gene Symbol: ERBB2
Synonyms: NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; MLN-19; c-ERB2; c-ERB-2; HER-2/neu; p185(erbB2); B2
Gene ID: 2064
UniProt ID: P04626
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1156-1255 of human HER2/ErbB2 (NP_004439.2).
Dilution: WB 1:100-1:500
Purification Method: Affinity purification
Concentration: 0.86 mg/ml
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-ERBB2 polyclonal antibody




Background

The gene ERBB2 encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.




Images

ERBB2 rabbit pAbWestern blot analysis of lysates from MCF7 cells, using [KO Validated] HER2/ErbB2 Rabbit pAb (A21768) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ERBB2 polyclonal antibodyWestern blot analysis of lysates from wild type(WT) and HER2/ErbB2 Rabbit pAb knockout (KO) HeLa cells, using [KO Validated] HER2/ErbB2 Rabbit pAb (A21768) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.



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