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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | GATA binding protein 3 |
| Gene Symbol: | GATA3 |
| Synonyms: | HDR; HDRS; A3 |
| Gene ID: | 2625 |
| UniProt ID: | P23771 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GATA3 (NP_001002295.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.26 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GATA3 polyclonal antibody |
Background
The gene GATA3 encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.
Images
 | Western blot analysis of lysates from wild type (WT) and GATA3 knockout (KO) 293T cells, using [KO Validated] GATA3 Rabbit pAb (A1638) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
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