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KO-Validated GCN1L1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | GCN1 activator of EIF2AK4 |
| Gene Symbol: | GCN1 |
| Synonyms: | GCN1L; GCN1L1; PRIC295; L1 |
| Gene ID: | 10985 |
| UniProt ID: | Q92616 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2462-2671 of human GCN1L1 (NP_006827.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 3.9 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GCN1 polyclonal antibody |
Background
Enables RNA binding activity and cadherin binding activity. Predicted to be involved in cellular response to leucine starvation and positive regulation of transcription from RNA polymerase II promoter in response to stress. Located in cytosol.
Images
_KO-WB_01.jpg?t=1709190799) | Western blot analysis of lysates from wild type (WT) and GCN1L1 knockout (KO) 293T cells, using [KO Validated] GCN1L1 Rabbit pAb (A19851) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30S. |
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