Reactivity: | Human |
Applications: | WB, IF/IC, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Monoclonal antibody |
Gene Name: | protein-coupled receptor kinase 2 |
Gene Symbol: | GRK2 |
Synonyms: | BARK1; ADRBK1; BETA-ARK1; K2 |
Gene ID: | 156 |
UniProt ID: | P25098 |
Clone ID: | 1E5K3 |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 590-689 of human GRK2 (P25098). |
Dilution: | WB 1:11000-1:110000; IF/IC 1:50-1:200 |
Purification Method: | Affinity purification |
Concentration: | 1 mg/mL |
Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-GRK2 monoclonal antibody |
Background
The gene GRK2 encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease.
Images
![]() | Western blot analysis of various lysates using GRK2 Rabbit mAb (A4443) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
![]() | Western blot analysis of lysates from wild type (WT) and GRK2 knockout (KO) HeLa cells, using [KO Validated] GRK2 Rabbit mAb (A4443) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
![]() | Immunofluorescence analysis of HeLa cells using GRK2 Rabbit mAb (A4443) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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