KO-Validated GRK2 Rabbit mAb (20 μl)

KO-Validated GRK2 Rabbit mAb (20 μl)

Brand: Abclonal
Cat. #: A4443
Availability: In Stock
$148.00
-+
Reactivity: Human    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: protein-coupled receptor kinase 2
Gene Symbol: GRK2
Synonyms: BARK1; ADRBK1; BETA-ARK1; K2
Gene ID: 156
UniProt ID: P25098
Clone ID: 1E5K3
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 590-689 of human GRK2 (P25098).
Dilution: WB 1:11000-1:110000; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 1 mg/mL
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-GRK2 monoclonal antibody




Background

The gene GRK2 encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease.




Images

GRK2 rabbit mAbWestern blot analysis of various lysates using GRK2 Rabbit mAb (A4443) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
GRK2 monoclonal antibodyWestern blot analysis of lysates from wild type (WT) and GRK2 knockout (KO) HeLa cells, using [KO Validated] GRK2 Rabbit mAb (A4443) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using GRK2 Rabbit mAb (A4443) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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