KO-Validated GSK3α Rabbit pAb (20 μl)

KO-Validated GSK3α Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A18655
Availability: In Stock
$128.00
-+
Reactivity: Human    
Applications: WB, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: glycogen synthase kinase 3 alpha
Gene Symbol: GSK3A
Synonyms: GSK3A; glycogen synthase kinase-3 alpha; 3α
Gene ID: 2931
UniProt ID: P49840
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-483 of human GSK3α (NP_063937.2).
Dilution: WB 1:500-1:2000
Purification Method: Affinity purification
Concentration: 0.72 mg/ml
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-GSK3A polyclonal antibody




Background

The gene GSK3A encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.




Images

GSK3A rabbit pAbWestern blot analysis of lysates from wild type (WT) and GSK3α knockout (KO) HeLa cells, using [KO Validated] GSK3α Rabbit pAb (18655) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot analysis of various lysates using [KO Validated] GSK3α Rabbit pAb (A18655) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.



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