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KO-Validated Histone H2A.Z Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | H2A.Z variant histone 1 |
| Gene Symbol: | H2AZ1 |
| Synonyms: | H2AZ; H2A.z; H2A/z; H2AFZ; H2A.Z-1; .Z |
| Gene ID: | 3015 |
| UniProt ID: | P0C0S5 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human H2AFZ (NP_002097.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.16 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-H2AZ1 polyclonal antibody |
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The gene H2AZ1 encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
Images
 | Western blot analysis of various lysates using [KO Validated] Histone H2A.Z Rabbit pAb (A6614) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type (WT) and Histone H2A.Z knockout (KO) 293T cells, using [KO Validated] Histone H2A.Z Rabbit pAb (A6614) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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