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KO-Validated CD168/RHAMM Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | hyaluronan mediated motility receptor |
| Gene Symbol: | HMMR |
| Synonyms: | CD168; IHABP; RHAMM; MM |
| Gene ID: | 3161 |
| UniProt ID: | O75330 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CD168/RHAMM (NP_001136028.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.46 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HMMR polyclonal antibody |
Background
The protein encoded by the gene HMMR is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Images
 | Western blot analysis of various lysates using [KO Validated] CD168/RHAMM Rabbit pAb (A12445) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Western blot analysis of lysates from wild type (WT) and CD168/RHAMM knockout (KO) HeLa cells, using [KO Validated] CD168/RHAMM Rabbit pAb (A12445) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit pAb (A12445). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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