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| Reactivity: | Human |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | heterogeneous nuclear ribonucleoprotein F |
| Gene Symbol: | HNRNPF |
| Synonyms: | HNRPF; mcs94-1; OK/SW-cl.23; PF |
| Gene ID: | 3185 |
| UniProt ID: | P52597 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HNRNPF (NP_001091678.1). |
| Dilution: | WB 1:1000-1:5000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.56 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HNRNPF polyclonal antibody |
Background
The gene HNRNPF belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Images
 | Western blot analysis of various lysates using [KO Validated] HNRNPF Rabbit pAb (A5505) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type(WT) and HNRNPF knockout (KO) 293T cells, using [KO Validated] HNRNPF Rabbit pAb (A5505) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of U2OS cells using [KO Validated] HNRNPF Rabbit pAb (A5505). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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