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KO-Validated HPCAL1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | hippocalcin like 1 |
| Gene Symbol: | HPCAL1 |
| Synonyms: | BDR1; HLP2; VILIP-3; L1 |
| Gene ID: | 3241 |
| UniProt ID: | P37235 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human HPCAL1 (NP_002140.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.44 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HPCAL1 polyclonal antibody |
Background
The protein encoded by the gene HPCAL1 is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Several alternatively spliced transcript variants encoding the same protein have been found for this gene.
Images
_KO-WB_01.jpg?t=1709606092) | Western blot analysis of lysates from wild type (WT) and HPCAL1 knockout (KO) HeLa cells, using [KO Validated] HPCAL1 Rabbit pAb (A19964) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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