KO-Validated Hsp90β Rabbit pAb (20 μl)

KO-Validated Hsp90β Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A18015
Availability: In Stock
$128.00
-+
Reactivity: Human    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: heat shock protein 90 alpha family class B member 1
Gene Symbol: HSP90AB1
Synonyms: HSP84; HSPC2; HSPCB; D6S182; HSP90B
Gene ID: 3326
UniProt ID: P08238
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90β (NP_031381.2).
Dilution: WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 1.08 mg/mL
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-HSP90AB1 polyclonal antibody




Background

The gene HSP90AB1 encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.




Images

HSP90AB1 rabbit pAbWestern blot analysis of lysates from wild type (WT) and HSP90AB1 knockout (KO) HeLa cells, using [KO Validated] Hsp90β Rabbit pAb (A18015) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.



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