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KO-Validated Hsp90β Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | heat shock protein 90 alpha family class B member 1 |
| Gene Symbol: | HSP90AB1 |
| Synonyms: | HSP84; HSPC2; HSPCB; D6S182; HSP90B |
| Gene ID: | 3326 |
| UniProt ID: | P08238 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90β (NP_031381.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.08 mg/mL |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HSP90AB1 polyclonal antibody |
Background
The gene HSP90AB1 encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.
Images
_KO-WB_01.jpg?t=1709182448) | Western blot analysis of lysates from wild type (WT) and HSP90AB1 knockout (KO) HeLa cells, using [KO Validated] Hsp90β Rabbit pAb (A18015) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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