KO-Validated Ki67 Rabbit PolymAb® (20 μl)

KO-Validated Ki67 Rabbit PolymAb® (20 μl)

Brand: Abclonal
Cat. #: A26755PM
Availability: In Stock
$148.00
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Reactivity: Human    
Applications: WB, IHC-P, IF/ICC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Monoclonal antibody    
Gene Name: marker of proliferation Ki-67
Gene Symbol: MKI67
Synonyms: KIA; MIB-; MIB-1; PPP1R105
Gene ID: 4288/17345
UniProt ID: P46013/E9PVX6
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2271-2370 of human Ki67 (NP_002408.3).
Purification Method: Affinity purification
Concentration: 0.72mg/ml
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-MKI67 monoclonal antibody




Background

Enables protein C-terminus binding activity. Involved in regulation of chromosome segregation and regulation of mitotic nuclear division. Located in chromosome; nuclear body; and nucleolus. Colocalizes with condensed chromosome. Implicated in Crohn's disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Biomarker of several diseases, including Barrett's esophagus; autoimmune disease of musculoskeletal system (multiple); endocrine gland cancer (multiple); gastrointestinal system cancer (multiple); and interstitial cystitis.




Images

MKI67 rabbit mAbWestern blot analysis of lysates from wild type (WT) and Ki67 knockout (KO) HeLa cells using [KO Validated] Ki67 Rabbit PolymAb® (A26755PM) at 1:1000 dilution incubated overnight at 4℃. HeLa cells and Ki67 knockout (KO) HeLa cells were treated by Nocodazole (1 μg/mL) at 37℃ for 24 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from RAW264.7 cells using [KO Validated] Ki67 Rabbit PolymAb® (A26755PM) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] Ki67 Rabbit PolymAb® (A26755PM) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Ki67 Rabbit PolymAb® (A26755PM) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] Ki67 Rabbit PolymAb® (A26755PM) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] Ki67 Rabbit PolymAb® (A26755PM) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] Ki67 Rabbit PolymAb® (A26755PM) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] Ki67 Rabbit PolymAb® (A26755PM) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.



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