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KO-Validated DJ-1/PARK7 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Parkinsonism associated deglycase |
| Gene Symbol: | PARK7 |
| Synonyms: | DJ1; DJ-1; GATD2; HEL-S-67p; K7 |
| Gene ID: | 11315 |
| UniProt ID: | Q99497 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human DJ-1/PARK7 (NP_009193.2). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.74 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-PARK7 polyclonal antibody |
Background
The product of the gene PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.
Images
 | Western blot analysis of various lysates using [KO Validated] DJ-1/PARK7 Rabbit pAb (A0987) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Western blot analysis of lysates from wild type (WT) and DJ-1/PARK7 knockout (KO) 293T cells, using [KO Validated] DJ-1/PARK7 Rabbit pAb (A0987) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of HeLa cells using [KO Validated] DJ-1/PARK7 Rabbit pAb (A0987). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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