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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ralA binding protein 1 |
| Gene Symbol: | RALBP1 |
| Synonyms: | RIP1; RLIP1; RLIP76; P1 |
| Gene ID: | 10928 |
| UniProt ID: | Q15311 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 396-655 of human RALBP1 (NP_006779.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 8.73 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-RALBP1 polyclonal antibody |
Background
RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).
Images
 | Western blot analysis of various lysates using [KO Validated] RALBP1 Rabbit pAb (A1140) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Western blot analysis of lysates from wild type (WT) and RALBP1 knockout (KO) HeLa cells, using [KO Validated] RALBP1 Rabbit pAb (A1140) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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