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| Reactivity: | Human |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ras homolog family member C |
| Gene Symbol: | RHOC |
| Synonyms: | H9; ARH9; ARHC; RHOH9; oC |
| Gene ID: | 389 |
| UniProt ID: | P08134 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RhoC (NP_786886.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.61 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-RHOC polyclonal antibody |
Background
The gene RHOC encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Images
 | Western blot analysis of lysates from wild type (WT) and RhoC knockout (KO) 293T cells, using [KO Validated] RhoC Rabbit pAb (A1062) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 300s. |
 | Western blot analysis of lysates from Rat heart , using [KO Validated] RhoC Rabbit pAb (A1062) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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