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KO-Validated SEC61B Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | SEC61 translocon subunit beta |
| Gene Symbol: | SEC61B |
| Synonyms: | SEC61B; 1B |
| Gene ID: | 10952 |
| UniProt ID: | P60468 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 3.6 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SEC61B polyclonal antibody |
Background
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.
Images
 | Western blot analysis of lysates from wild type (WT) and SEC61B knockout (KO) 293T cells, using [KO Validated] SEC61B Rabbit pAb (A15788) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using [KO Validated] SEC61B Rabbit pAb (A15788) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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