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| Reactivity: | Human, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | SMAD family member 5 |
| Gene Symbol: | SMAD5 |
| Synonyms: | DWFC; JV5-1; MADH5; d5 |
| Gene ID: | 4090 |
| UniProt ID: | Q99717 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-260 of human Smad5 (NP_005894.3). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SMAD5 monoclonal antibody |
Background
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from wild type(WT) and Smad5 knockout (KO) 293T(KO) cells, using [KO Validated] Smad5 Rabbit mAb (A22749) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
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