KO-Validated N-terminal alpha-synuclein mAb (20 μl)

Brand: Abclonal
Cat. #: A27117
Availability: In Stock

$141.00

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Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, IF/IC, ELISA
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	Synuclein alpha
Gene Symbol:	SNCA
Synonyms:	NACP; PARK1; α-Synuclein; SNCA
Gene ID:	6622
UniProt ID:	P37840
Immunogen:	A synthetic peptide corresponding to a sequence corresponding to amino acids 40-50 of human alpha-synuclein(NP_000336.1).
Dilution:	WB 1:5000-1:30000; IF/IC 1:200-1:500
Purification Method:	Affinity purification
Concentration:	1.78 mg/ml
Buffer:	PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-SNCA monoclonal antibody

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Alpha-synuclein is a neuronal protein that plays many roles in synaptic activity such as membrane trafficking of synaptic vesicles and neurotransmitter release. Multiplications in SNCA have been implicated in genetic forms of Parkinson disease and pathological post-translational modifications of alpha-synuclein are a hallmark of Parkinson’s disease. These modifications include phosphorylation, nitration, acetylation, truncation, and aggregation.

Images

	Western blot analysis of lysates from wild type (WT) and Alpha synuclein knockout (KO) aSyn cells using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117) at 1:5000 dilution . Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of various lysates using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117) at 1:5000 dilution . Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): T-47D Exposure time: 60s.
	Western blot analysis of various lysates using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117) at 1:5000 dilution . Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Confocal imaging of Mouse primary hippocampal neuron cells(Wild type) and Mouse primary hippocampal neuron cells(SNCA KO) using N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117, dilution 1:500) followed by a further incubation with AF488 conjugated donkey Anti-Rabbit (H+L) (A-21206, dilution 1:500) (Green). The cells were counterstained with total aSyn antibody and MAP2 antibody followed by incubation with AF647 conjugated Donkey Anti-mouse IgG (H+L) Ab (A-31571) (Red) and AF568 conjugated Donkey Anti-chicken IgG (H+L) Ab (703-545-155, dilution 1:400) (Grey).DAPI was used for nuclear staining (Blue). Objective: 40x.