KO-Validated STRAP Rabbit pAb (20 μl)

KO-Validated STRAP Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A21351
Availability: In Stock
$128.00
-+
Reactivity: Human    
Applications: WB, IF/IC, IP, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: serine/threonine kinase receptor associated protein
Gene Symbol: STRAP
Synonyms: MAWD; PT-WD; UNRIP; AP
Gene ID: 11171
UniProt ID: Q9Y3F4
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human STRAP (NP_009109.3).
Dilution: WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 2.17 mg/mL
Buffer: PBS with 0.02% sodium azide, 50% glycerol ,pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-STRAP polyclonal antibody




Background

Enables RNA binding activity. Involved in maintenance of gastrointestinal epithelium; negative regulation of transforming growth factor beta receptor signaling pathway; and spliceosomal snRNP assembly. Located in cytosol. Part of SMN complex. Implicated in adenocarcinoma; colorectal carcinoma; large cell carcinoma; lung carcinoma; and squamous cell neoplasm.




Images

STRAP rabbit pAbWestern blot analysis of lysates from wild type(WT) and STRAP knockout (KO) HeLa cells, using STRAP Rabbit pAb (A21351) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
STRAP polyclonal antibodyImmunofluorescence analysis of H9C2 cells using STRAP Rabbit pAb (A21351) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Anti-STRAP antibodyImmunofluorescence analysis of L929 cells using STRAP Rabbit pAb (A21351) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using STRAP Rabbit pAb (A21351) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg STRAP antibody (A21351). Western blot was performed from the immunoprecipitate using STRAP antibody (A21351) at a dilution of 1:1000.



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