Reactivity: | Human |
Applications: | WB, IF/IC, IP, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | serine/threonine kinase receptor associated protein |
Gene Symbol: | STRAP |
Synonyms: | MAWD; PT-WD; UNRIP; AP |
Gene ID: | 11171 |
UniProt ID: | Q9Y3F4 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human STRAP (NP_009109.3). |
Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
Purification Method: | Affinity purification |
Concentration: | 2.17 mg/mL |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-STRAP polyclonal antibody |
Background
Enables RNA binding activity. Involved in maintenance of gastrointestinal epithelium; negative regulation of transforming growth factor beta receptor signaling pathway; and spliceosomal snRNP assembly. Located in cytosol. Part of SMN complex. Implicated in adenocarcinoma; colorectal carcinoma; large cell carcinoma; lung carcinoma; and squamous cell neoplasm.
Images
![]() | Western blot analysis of lysates from wild type(WT) and STRAP knockout (KO) HeLa cells, using STRAP Rabbit pAb (A21351) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![]() | Immunofluorescence analysis of H9C2 cells using STRAP Rabbit pAb (A21351) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunofluorescence analysis of L929 cells using STRAP Rabbit pAb (A21351) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunofluorescence analysis of U2OS cells using STRAP Rabbit pAb (A21351) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg STRAP antibody (A21351). Western blot was performed from the immunoprecipitate using STRAP antibody (A21351) at a dilution of 1:1000. |
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