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| Reactivity: | Human |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Tumor protein p53 |
| Gene Symbol: | TP53 |
| Synonyms: | P53; BCC7; LFS1; BMFS5; TRP53; 53 |
| Gene ID: | 7157 |
| UniProt ID: | P04637 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (NP_000537.3). |
| Dilution: | WB 1:1000-1:2000; IHC 1:50-1:200; IF/IC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TP53 monoclonal antibody |
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Images
 | Western blot analysis of lysates from wild type (WT) and p53 knockout (KO) 293T cells using [KO Validated] p53 Rabbit mAb (A19585) at 1:59000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KO Validated] p53 Rabbit mAb (A19585). Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit mAb (A19585) at a dilution of 1:1000. |
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