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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | X-linked inhibitor of apoptosis |
| Gene Symbol: | XIAP |
| Synonyms: | API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3; AP |
| Gene ID: | 331 |
| UniProt ID: | P98170 |
| Clone ID: | 5S5Q7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human XIAP (NP_001158.2). |
| Dilution: | WB 1:2000-1:6000 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-XIAP monoclonal antibody |
Background
The gene XIAP encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.
Images
 | Western blot analysis of various lysates, using [KO Validated] XIAP Rabbit mAb (A20846) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Western blot analysis of lysates from wild type (WT) and XIAP knockout (KO) HeLa cells, using [KO Validated] XIAP Rabbit mAb (A20846) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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