KO-Validated XIAP Rabbit pAb (20 μl)

KO-Validated XIAP Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A6869
Availability: In Stock
$128.00
-+
Reactivity: Human    
Applications: WB, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: X-linked inhibitor of apoptosis
Gene Symbol: XIAP
Synonyms: API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3; AP
Gene ID: 331
UniProt ID: P98170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 198-497 of human XIAP (NP_001158.2).
Dilution: WB 1:500-1:2000
Purification Method: Affinity purification
Concentration: 0.91 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-XIAP polyclonal antibody




Background

The gene XIAP encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.




Images

XIAP rabbit pAbWestern blot analysis of various lysates using [KO Validated] XIAP Rabbit pAb (A6869) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of lysates from wild type (WT) and XIAP knockout (KO) HeLa cells, using [KO Validated] XIAP Rabbit pAb (A6869) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of various lysates using [KO Validated] XIAP Rabbit pAb (A6869) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.



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