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| Reactivity: | H,M,R & H |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Proliferation marker protein Ki-67 |
Synonyms: | MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105 |
Immunogen: | Human recombinant Ki67 protein expressed in HEK293 cells |
Uniprot ID: | P46013 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 359 kDa / 359 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa, HepG2, A-431, HCT116, MCF7 |
IHC/IF | Human | 1: 500-1: 1500 | colon, tonsil |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface.
Images
| Western blot analysis of Ki67 (GB121142) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human colon using ki67 (GB121142) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using ki67 (GB121142) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
