Reactivity: | M |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Prolyl 3-hydroxylase 2 |
Synonyms: | leprecan like 1, Leprecan like protein 1, LEPREL1, MLAT4, P3H2, Prolyl 3 hydroxylase 2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse P3H2 |
Uniprot ID: | Q8CG71 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 80 kDa / 90 kDa |
Product Usage Information
WB | Mouse | 1: 1500-1: 2000 | kidney |
Background
Prolyl 3-hydroxylase that catalyzes the post-translational formation of 3-hydroxyproline on collagens . Contributes to proline 3-hydroxylation of collagen COL4A1 and COL1A1 in tendons, the eye sclera and in the eye lens capsule . Has high activity with the type IV collagen COL4A1, and lower activity with COL1A1. Catalyzes hydroxylation of the first Pro in Gly-Pro-Hyp sequences where Hyp is 4-hydroxyproline. Has no activity on substrates that have proline instead of 4-hydroxyproline in the third position (By similarity).
Images
Western blot analysis of P3H2 (GB115118) at dilution of 1: 1500 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |