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| Key Features: | • Rapid: DNA purification process in less than 20 min post-lysis. • Safe: No Phenol/chloroform extractions. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: | LiPureTM Magbead Tissue DNA Purification Kit provides a rapid and reliable isolation of high-quality genomic DNA from fresh or frozen animal tissue samples. This kit can be processed manually or on an automated platform. This system combines the reversible nucleic acid binding properties of paramagnetic particles with the time-proven efficiency of LifeSct’s buffer chemistries to provide a fast and convenient method to isolate DNA from a variety of samples. Phenol/chloroform extraction, and time-consuming steps such as precipitation with isopropanol or ethanol have been eliminated. Utilizing paramagnetic particles provides high-quality DNA that is suitable for direct use in most downstream applications such as Next Generation Sequencing, qPCR, PCR, and microarrays. |
| Applications: | Total DNA isolation from fresh and frozen animal tissues. |
| Technical Specifications: | • Sample Input: 10-25 mg tissues, 5×106 cultured cells • Elution Volume: 50-100 μl • Technology: Magnetic Beads |
| Sample Source: | Fresh and frozen tissues, paraffin-embedded tissues, cells, mouse tail snips, blood, and buccal swabs. |
| Component: | 1. Buffer ATL: 15 ml 2. Buffer DL: 15 ml 3. Buffer ACW1: 15 ml 4. Buffer ACW2: 15 ml 5. Buffer AE: 15 ml 6. Proteinase K: 1 ml, 20 mg/ml 7. MagBinding Beads: 2.5 ml |
| Storage: | Bufferat RT; Proteinase K at -20°C; MagBinding Beads at 2-8°C |
| Documents: | Manual |
Case Study
Figure 1. Plasma samples were purified using LiPureTM cfDNA Purification Kit. The purified cfDNA samples were loaded on a D100 high-sensitive tape on TapeStation® 2200.
Table 1. Genomic DNA yield and quality using LiPureTM cfDNA Purification Kit
