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| Key Features: |
• Rapid: DNA purification process in less than 2 hr. • Safe: No Phenol/chloroform extractions. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: |
LiPureTM Magbead cfDNA Purification Kit provides a rapid and reliable isolation of circulating DNA from plasma, serum, and other cellular body fluids. The kit can be processed manually or on an automated platform. The uniquely formulated lysis and binding buffers allow samples to be completely lysed, and binded to magnetic beads. The impurities such as cellular debris, hemoglobin, and other proteins are washed away using washing buffer. The isolated circulating DNA is ready for applications such as PCR, microarrays, and next generation sequencing. |
| Applications: |
Circulating DNA isolation from plasma, serum, and other cellular body fluids. |
| Technical Specifications: |
• Sample Input: 1-4 ml • Elution Volume: 20-50 μl • Technology: Magnetic Beads |
| Sample Source: |
plasma, serum, and other cellular body fluids. |
| Component: |
1. Buffer ACL: 50 ml 2. Buffer ACB: 50 ml 3. Buffer ACW1: 15 ml 4. Buffer ACW2: 10 ml 5. Buffer AE: 15 ml 6. Proteinase K: 2×1.3 ml, 20 mg/ml 7. Carrier RNA: 250 µl, 0.2 mg/ml 8. MagBinding Beads: 2.5 ml |
| Storage: |
Buffer & tube at RT; Proteinase K & Carrier RNA at -20°C |
| Documents: |
Manual |
Case Study
Figure 1. Plasma samples were purified using LiPureTM cfDNA Purification Kit from serum/plasma. The purified cfDNA samples were loaded on a D100 high-sensitive tape on TapeStation® 2200.