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| Key Features: | • Rapid: DNA purification process in less than 30 min. • Safe: No organic extraction, no ethanol precipitation. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: | LiPureTM Stool DNA Purification Kit provides fast and easy purification of total DNA from fresh or frozen stool samples. Up to 200 mg of stool samples can be processed in less than 30 minutes. The specially formulated lysis buffer allows direct cell lysis and removes DNA-degrading substances and PCR inhibitors present in the stool sample by centrifugation. The DNA in the supernatant is then purified on the DNA spin column. Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. The purified DNA is of high quality and well suited for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion. |
| Applications: | Total DNA isolation from fresh or frozen stool samples. |
| Technical Specifications: | • Sample Input: 200 mg stoll sample • Elution Volume: 50-100 μl • Technology: Silica spin column |
| Sample Source: | Stool |
| Component: | 1. Buffer ASL: 50 ml 2. Buffer DL: 25 ml 3. Buffer AW1: 15 ml 4. Buffer AW2: 15 ml 5. Buffer AE: 15 ml 6. Proteinase K: 1 ml, 20 mg/ml 7. Mini Column: 50 8. 2 ml Collection Tube: 50 |
| Storage: | Buffer & tube at RT; Proteinase K at -20°C |
| Documents: | Manual |
Case Study
Figure 1. Genomic DNA from three human stool samples was isolated using LiPureTM Stool DNA Purification Kit. Eluted DNA was analyzed on a 1% agarose gel.