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| Key Features: |
• Rapid: DNA purification process in less than 20 min post-lysis. • Safe: No Phenol/chloroform extractions. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: |
LiPure™ Tissue DNA Purification Kit provides a rapid and easy method for the isolation of DNA from fresh or frozen animal tissue samples. The DNA purification process is simplified with Spin Column technology into four quick “lyse-bind-wash-elute” steps and can be accomplished in less than 20 minutes post-lysis. This convenient spin-column format avoids time-consuming steps like alcohol precipitation, use of toxic compounds such as phenol and chloroform and allows for multiple samples to be processed in parallel. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion. |
| Applications: |
Total DNA isolation from fresh and frozen animal tissues. |
| Technical Specifications: |
• Sample Input: 10-25 mg tissues, 5×106 cultured cells • Elution Volume: 30-100 μl • Technology: Silica spin column |
| Sample Source: |
Fresh and frozen tissues, paraffin-embedded tissues, cells, mouse tail snips, blood, and buccal swabs. |
| Component: |
1. Buffer ATL: 15 ml 2. Buffer DL: 15 ml 3. Buffer ACW1: 15 ml 4. Buffer ACW2: 15 ml 5. Buffer AE: 15 ml 6. Proteinase K: 1 ml, 20 mg/ml 7. Mini Column: 50 8. 2 ml Collection Tube: 50 |
| Storage: |
Buffer & tube at RT; Proteinase K at -20°C |
| Documents: |
Manual |
Case Study
Table 1. Genomic DNA yield and quality using LiPureTM Tissue DNA Purification Kit.
