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| Key Features: | • Rapid: DNA purification process in less than 20 min post-lysis. • Safe: No organic extraction, no ethanol precipitation. • High-quality: DNA is suitable for a variety of downstream applications. |
| Description: | LiPureTM Universal DNA Purification Kit is designed for rapid purification of genomic DNA from a variety of sample sources including fresh or frozen animal tissues and cells, blood, serum, plasma, saliva, swab, sperm, blood spot. The DNA purification process is simplified with Spin Column technology into four quick “lyse-bind-wash-elute” steps and can be accomplished in less than 20 minutes post-lysis. This convenient spin-column format avoids time-consuming steps like alcohol precipitation, use of toxic compounds such as phenol and chloroform and allows for multiple samples to be processed in parallel. DNA purified using this kit is ready for most downstream applications such as PCR, sequencing, genotyping, southern blot analysis and restriction enzyme digestion. |
| Applications: | Genomic DNA isolation from a variety of sample sources including fresh or frozen animal tissues and cells, blood, serum, plasma, saliva, swab, sperm, blood spot. |
| Technical Specifications: | • Elution Volume: 50-100 μl • Technology: Silica spin column |
| Sample Source: | Animal tissues and cells, blood, serum, plasma, saliva, swab, sperm, blood spot. |
| Component: | 1. Buffer ATL: 15 ml 2. Buffer DL: 15 ml 3. Buffer AW1: 15 ml 4. Buffer AW2: 15 ml 5. Buffer AE: 15 ml 6. Proteinase K: 1 ml, 20 mg/ml 7. Mini Column: 50 8. 2 ml Collection Tube: 50 |
| Storage: | Buffer & tube at RT; Proteinase K at -20°C |
| Documents: | Manual |
Case Study
Figure 1. Genomic DNA from different samples was isolated using LiPureTM Universal DNA Purification Kit. Eluted DNA was analyzed on a 1% agarose gel.