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The LiQuant™ dsDNA HS Assay Kit is meticulously designed for effortless and precise quantification of double-stranded DNA (dsDNA). This kit excels in selectivity for dsDNA over RNA and surpasses traditional DNA quantitation methods in terms of stability, linear dynamic range, and sensitivity.
Comprising a concentrated quantitation reagent, dilution buffer, and pre-diluted dsDNA standards, the kit simplifies the process of dsDNA measurement. To use, just dilute the reagent with the provided buffer, add your sample—accepting any volume from 1 µl to 20 µl—and measure the fluorescence with a fluorescence plate reader or fluorometer. The assay demonstrates robust tolerance to common contaminants, including proteins, salts, solvents, detergents, and free nucleotides, ensuring reliable results even in the presence of these interfering substances.
Furthermore, the assay's versatility allows it to be adapted for use in microplates, tubes, or cuvettes, making it a flexible tool for various experimental setups and high-throughput applications.
Key Features
• Higher sensitivity: 100 times more sensitive than NanoDrop.
• Broad dynamic range: over four orders of magnitude.
• More accuracy: unaffected by the presence of proteins, free nucleotides.
• Easy to use: mix-and-read format.
• Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit.
Applications
dsDNA quantitation of PCR products, viral DNA and samples for subcloning and Next Gen Sequencing
Specifications
1. Sample Type: dsDNA
2. Platform: Fluorescence plate reader or fluorometer
3. Assay: dsDNA Quantitation, high sensitivity
4. Linear Range: 0.2-100 ng dsDNA
Components
1. LiQuant™ dsDNA BR Reagent: 200 µl
