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LiQuant™ ssDNA Assay Kit is ideal for quantitating ssDNA or oligonucleotides. The kit is not selective for ssDNA over dsDNA or RNA, but it will not detect contaminating protein or nucleotides. The kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted ssDNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer. The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.
Key Features
• Higher sensitivity: 100 times more sensitive than NanoDrop.
• Broad dynamic range: over four orders of magnitude.
• More accuracy: unaffected by the presence of proteins, free nucleotides.
• Easy to use: mix-and-read format.
• Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit.
Applications
ssDNA quantitation of oligo, primers, denatured DNA, PCR products
Specifications
1. Sample Type: ssDNA
2. Platform: Fluorescence plate reader or fluorometer
3. Linear Range: 1-200 ng ssDNA
Components
1. LiQuant™ ssDNA Reagent: 200 µl
2. LiQuant™ ssDNA Buffer: 50 ml
3. ssDNA Standard #1: 200 µl
4. ssDNA Standard #2: 200 µl
Storage
Store at 2-8°C and protect from light.
Case Study
Left: Quantitation of ssDNA with LiQuant™ ssDNA Assay Kit using fluorescence plate reader. Right: Quantitation of ssDNA with LiQuant™ ssDNA Assay Kit using Qubit® Fluorometer.
