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LiScript™ Fast RT Master Mix is a reagent for fast reverse transcription. 5× LiScript RT Master Mix contains all the components needed for first strand cDNA synthesis. The efficiency and yield of cDNA first-strand synthesis are higher, and the first strand of cDNA can be synthesized using pg total RNA or mRNA. The reaction can be completed in 15 minutes. This kit is suitable for the high throughout synthesis of first-strand cDNA and subsequent RT-PCR, RT-qPCR, and construction of full-length cDNA libraries.
Key Features
• Ready and easy to use: The reaction will be initiate by adding water and RNA template to the master mix.
• Rapid reverse transcription: It takes only 15 minutes to obtain the first strand of cDNA.
• High sensitivity: pg of total RNA or mRNA can be used as template.
• High efficiency: Above 90% RNA will be reverse transcripted.
Components
1. 5× LiScript RT Master Mix: 100 µl
2. RNase-Free Water: 500 µl
Storage
Store at -20°C.
