LiScript™ First-Strand cDNA Synthesis Kit (100 rxn)

• Efficient reverse transcription efficiency: novel mutation sites greatly increase enzyme activity and obtain higher yields of cDNA.

• Good extension ability: point mutations eliminate RNase H activity and improve the affinity of reverse transcriptase and RNA template to obtain cDNA up to 12 kb.

• High sensitivity: The first strand cDNA can be synthesized using pg total RNA or mRNA template.

• Convenient: All reverse transcription reagents are already provided in the kit. Reverse transcription can be easily accomplished by simply adding a template.

LiScriptTM First-Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit designed for the first step of two-step RT-PCR. The reverse transcriptase used in the kit is a novel high-efficiency reverse transcriptase derived from M-MLV. The novel mutation site greatly enhances the transcriptional activity of the enzyme. The efficiency and yield of cDNA first-strand synthesis are higher, and this kit can be used with as little as pg level of total RNA or mRNA. The point mutation eliminates RNase H activity, improves the elongation ability of the enzyme, and effectively improves the affinity of the reverse transcriptase to the RNA template, resulting in a first strand cDNA of up to 12 kb. Optimized RT Buffers allow a wider range of applications for reverse transcriptase and are highly compatible with subsequent PCR and quantitative PCR experiments. The kit is equipped with all reverse transcription reagents and is easy to use. It is suitable for the synthesis of first-strand cDNA and subsequent RT-PCR, RT-qPCR, and construction of full-length cDNA libraries.