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| Key Features: | • Rapid genomic DNA deletion: With the gDNA remover, it takes only 2 minutes to remove genomic DNA. • Rapid reverse transcription: It takes only 15 minutes to obtain the first strand of cDNA. • Easy to use: RT Master Mix contains all the components needed for reverse transcription and ready to use. • High sensitivity: pg of total RNA or mRNA can be used as template. • High efficiency of reverse transcription efficiency: the novel mutation site enhancers the activity of the enzyme, to increase the yield of cDNA. |
| Description: | LiScriptTM Fast RT Master Mix (+gDNA wiper) is a kit for reverse transcription after removal of genomic DNA. The kit removes genomic DNA in 2 minutes at 42°C. Meanwhile, because the reverse transcription reagents contain components that inhibit gDNA Remover, the sample processed by gDNA remover can be directly used for reverse transcription reaction to synthesize cDNA. This kit contains a novel high-performance reverse transcriptase. 5× LiScriptTM RT Master Mix contains all the components needed for reverse transcription. The novel mutation site greatly enhances the transcriptional activity of the enzyme. The efficiency and yield of cDNA first-strand synthesis are higher, and the first strand of cDNA can be synthesized using pg total RNA or mRNA. If the cDNA is used for downstream qPCR, the reverse transcription reaction can be completed in 15 minutes. This kit is suitable for the synthesis of first-strand cDNA and subsequent RT-PCR, RT-qPCR, and construction of full-length cDNA libraries. |
| Component: | 1. 5× gDNA Remover Mix: 100 µl 2. 5× LiScriptTM RT Master Mix: 400 µl 3. RNase-Free Water: 1.5 ml |
| Storage: | At -20°C |
| Documents: | Manual MSDS |
